P01.49. Development of quality control methods for some Indian integrative medicines by use of markers

Methods Stated formulations contain the common crude drug named piper longum. As we know that piperine is the key active plant ingredient of the drug and responsible for its therapeutic activity, both formulations have been standardized through high performance thin layer chromatography with respect to this marker compound (piperine). The stationary phase used was precoated silica gel 60F 254. The mobile phase containing toluene and methanol in proportion of 80:10 v/v was used to separate the spot of piperine. The detection of the spot was carried out at 345.5 nm. The proposed HPTLC method can be used for quality control of the raw material as well as the formulations.